Mycobiont and Whole Thallus Cultures of Roccella Montagnei Bél. For the Biosynthesis of Secondary Compounds

  • G. N. Hariharan Lichen Ecology and Bioprospecting Laboratory, M.S. Swaminathan Research Foundation, Taramani, Chennai 600 113, India.
  • S. Muthukumar Lichen Ecology and Bioprospecting Laboratory, M.S. Swaminathan Research Foundation, Taramani, Chennai 600 113, India.
  • S. Karthik Lichen Ecology and Bioprospecting Laboratory, M.S. Swaminathan Research Foundation, Taramani, Chennai 600 113, India.
DOI https://doi.org/.

Abstract

The mycobiont and whole thallus cultures of Roccella montagnei Bel. were established using soredia as an inoculum.The mycobiont
cultures showed optimum growth, biomass and biosynthesis of compounds in Lilly and Barnett medium with glucose as a carbon source,
micronutrients and vitamins. After the incubation period of 180 days, the cultures were harvested, and their biomass and secondary
compound profiles were analysed. The HPTLC chromatogram of the acetone extract of the NT and mycobiont cultures revealed erythrin
as the major biosynthesized compound in both and identified as a key biosynthate by R. montagnei. Further, the NT biosynthesized 5
additional compounds and the mycobiont cultures biosynthesized 6 additional compounds. The molecular identity of the cultured
mycobiont was confirmed using nuclear ribosomal Internal Transcribed Spacer (ITS) as well as the secondary chemistry. Lichen
compound erythrin was identified as a key biosynthate by the cultures.
Keywords: Roccella montagnei, Mycobiont, Whole thallus culture, Soredia, Erythrin

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