Evaluation of Phytochemical Constituents and Antioxidant activity of the Roccella montagnei

Abstract

The purpose of the present study is to evaluate the phytochemical constituents of Roccella
montagnei, qualitatively and quantitatively, found commonly in the Coromandel Coast, Tamil
Nadu India. In the study we also executed phenolic acids identification by GCMS and more over
estimation of their antioxidant potential for the very first time. As the several medicinal values
have been reported previously hence this study was to find out the governing factors for these
medicinal properties in Roccella montagnei. The Plant material was extracted with different
solvent i.e. methanol, Hexane, chloroform, ethyl acetate and water. Phytochemical screening,
Total Phenolic and Flavonoid content were analysed in all the solvent extracts the antioxidant
activity was achieved by 2, 2-diphenyl-2- picrylhydrazyl (DPPH) method. Some commonly
occurring phenolic were identified and quantified by GC-MS. Solvent extracts were
phytochemically screened out. Total Phenolic and Flavonoid contents were estimated in different
solvents extracts. Phenolic content were found in a higher amount comparative to flavonoids.
Similarly comparative Antioxidant activity of various solvent extracts were determined in which
methanolic extract showed marginally well antioxidant activity. Eight phenolic acids were identified
by GC-MS as gallic acid, syringic acid, p-coumaric acid, caffeic acid, ferulic acid, rutin, chlorogenic
acid and 4-hydroxy benzoic acid. Overall, present study provides a suitable criterion to qualitative
and quantitative evaluation of Roccella montagnei lichen sample for new vista for the exploitation
of this of Roccella for the development of herbal formulations.